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cmv promoter expressing spycas9  (Addgene inc)


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    Structured Review

    Addgene inc cmv promoter expressing spycas9
    Cmv Promoter Expressing Spycas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cmv+promoter+expressing+spycas9/pCSDest2-SpCas9-NLS-3XHA-NLS+(Plasmid+%2369220)/us12553039-552-6-10
    Average 92 stars, based on 7 article reviews
    cmv promoter expressing spycas9 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    other:

    Article Title: CRISPR-enhanced human adipocyte browning as cell therapy for metabolic disease
    Article Snippet: Oligomycin was used at 2.5 μM final concentration, FCCP at 5 μM and 10 μM L-(−)-Norepinephrine (+)-bitartrate salt (Sigma #N5785) was added where indicated.

    Article Title: Targeting Nrip1 to alleviate metabolic disease
    Article Snippet: The integrity of the library was confirmed by Agilent Tapestation using Agilent High Sensitivity D1000 ScreenTape kit.

    Article Title: CRISPR-enhanced human adipocyte browning as cell therapy for metabolic disease.
    Article Snippet: Membranes were washed in TBST, followed by incubating with Perkin Elmer Western Lightning Enhance ECL.

    Plasmid Preparation:

    Article Title: CRISPR-enhanced human adipocyte “browning” as cell therapy for metabolic disease
    Article Snippet: .. The pCS2-Dest plasmid with CMV promoter expressing SpyCas9 (Addgene # 69220), and sgRNA expressing plasmid (Addgene #52628) were a gift from Dr. Scot Wolfe lab. To clone NRIP1 targeting and non-targeting sgRNAs, oligo spacers with BfuAI overhangs (purchased from IDT) were annealed and cloned into the BfuAI-digested sgRNA plasmid. .. Lonza pmaxGFP LOT 2-00096 was used to test transfection of these plasmids in various concentrations to determine the efficient dosage range (0.5–1.5μg) and the electroporation conditions (1350 V, 30ms, 1 pulse) for the delivery and GFP expression was evaluated with EVOS FL fluorescent microscope (Thermo Fisher Scientific).

    Article Title: Targeting Nrip1 to alleviate metabolic disease
    Article Snippet: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041). .. The pCS2-Dest plasmid with CMV promoter expressing SpyCas9 (Addgene #69220), and sgRNA expressing plasmid (Addgene #52628) were a gift from Dr. Scot Wolfe lab. To clone NRIP1 targeting and non-targeting sgRNAs, oligo spacers with BfuAI overhangs (purchased from IDT) were annealed and cloned into the BfuAI-digested sgRNA plasmid. .. Three days after transfection, genomic DNA was isolated using the DNeasy Blood and Tissue kit (Qiagen) according to manufacturer's instructions.

    Expressing:

    Article Title: CRISPR-enhanced human adipocyte “browning” as cell therapy for metabolic disease
    Article Snippet: .. The pCS2-Dest plasmid with CMV promoter expressing SpyCas9 (Addgene # 69220), and sgRNA expressing plasmid (Addgene #52628) were a gift from Dr. Scot Wolfe lab. To clone NRIP1 targeting and non-targeting sgRNAs, oligo spacers with BfuAI overhangs (purchased from IDT) were annealed and cloned into the BfuAI-digested sgRNA plasmid. .. Lonza pmaxGFP LOT 2-00096 was used to test transfection of these plasmids in various concentrations to determine the efficient dosage range (0.5–1.5μg) and the electroporation conditions (1350 V, 30ms, 1 pulse) for the delivery and GFP expression was evaluated with EVOS FL fluorescent microscope (Thermo Fisher Scientific).

    Article Title: Targeting Nrip1 to alleviate metabolic disease
    Article Snippet: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041). .. The pCS2-Dest plasmid with CMV promoter expressing SpyCas9 (Addgene #69220), and sgRNA expressing plasmid (Addgene #52628) were a gift from Dr. Scot Wolfe lab. To clone NRIP1 targeting and non-targeting sgRNAs, oligo spacers with BfuAI overhangs (purchased from IDT) were annealed and cloned into the BfuAI-digested sgRNA plasmid. .. Three days after transfection, genomic DNA was isolated using the DNeasy Blood and Tissue kit (Qiagen) according to manufacturer's instructions.

    Clone Assay:

    Article Title: CRISPR-enhanced human adipocyte “browning” as cell therapy for metabolic disease
    Article Snippet: .. The pCS2-Dest plasmid with CMV promoter expressing SpyCas9 (Addgene # 69220), and sgRNA expressing plasmid (Addgene #52628) were a gift from Dr. Scot Wolfe lab. To clone NRIP1 targeting and non-targeting sgRNAs, oligo spacers with BfuAI overhangs (purchased from IDT) were annealed and cloned into the BfuAI-digested sgRNA plasmid. .. Lonza pmaxGFP LOT 2-00096 was used to test transfection of these plasmids in various concentrations to determine the efficient dosage range (0.5–1.5μg) and the electroporation conditions (1350 V, 30ms, 1 pulse) for the delivery and GFP expression was evaluated with EVOS FL fluorescent microscope (Thermo Fisher Scientific).

    Article Title: Targeting Nrip1 to alleviate metabolic disease
    Article Snippet: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041). .. The pCS2-Dest plasmid with CMV promoter expressing SpyCas9 (Addgene #69220), and sgRNA expressing plasmid (Addgene #52628) were a gift from Dr. Scot Wolfe lab. To clone NRIP1 targeting and non-targeting sgRNAs, oligo spacers with BfuAI overhangs (purchased from IDT) were annealed and cloned into the BfuAI-digested sgRNA plasmid. .. Three days after transfection, genomic DNA was isolated using the DNeasy Blood and Tissue kit (Qiagen) according to manufacturer's instructions.



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